Figure 2.
Chronic engagement of NKG2D in vivo does not affect NKp46-mediated cytotoxicity. (A,B) Redirected cytolysis assay with antibody-loaded P815 cells and in vivo preactivated splenic NK cells [poly(I:C) injection 16 h prior to isolation] from H2-Kb-MICA mice and non-transgenic littermates (non-tgLM), respectively. Data represent means with SDs. One representative out of at least four independent experiments is shown. Two-way ANOVA with a Sidak posttest was applied. (C) Degranulation (CD107a surfacing) of in vivo preactivated splenic NK cells after 4 h stimulation with plate-bound anti-NKp46 antibodies. Medium or stimulation with PMA/ionomycin served as controls. (A–C) Data represent means of triplicates with SDs. One representative out of at least three independent experiments is shown. Statistical significance was assessed with two-way ANOVA with Sidak posttest.