Figure 4.

Chronic engagement of NKG2D in vivo does not affect tumor growth of B16F10 melanoma. (A,B) H2-K^b-MICA mice (red lines/dots) and non-transgenic littermates (non-tgLM) (black lines/dots) were inoculated subcutaneously with (A) 1 × 10⁴ B16F10-MICA*01 or (B) 1 × 10⁴ B16F10-mock cells. Tumor size was measured every second day using a metric caliper and mice with a tumor size exceeding 14 mm were euthanized. Tumor growth (left panels), tumor growth rate (middle), and survival (right) is shown for seven mice per group. Growth rates of tumors were calculated as described in “Materials and Methods” section. Statistical significance of differences in tumor growth rate or in survival was calculated using unpaired t-test or log-rank (Mantel–Cox) test, respectively.