Figure 1.

Oxygen and glucose deprivation (OGD) induces inward currents and membrane depolarization in Bergmann glial cells. (A) Current response of a Bergmann cell to 30 min of OGD (I_OGD). The membrane potential is held at −70 mV. At the bottom, current traces from several Bergmann cells (n = 19) are averaged every minute. (B) Representative current clamp recording (V_OGD) from a Bergmann cell during OGD. No current was injected in these experiments. Note that Bergmann glia depolarizes gradually during OGD while, in the post-OGD phase, the recovery toward the baseline membrane potential is faster. The time-dependent changes in membrane potential from n = 12 Bergmann cells during OGD is shown at the bottom. (C) Time intervals between the beginning of the OGD and the first peak of the response (dashed lines, “time to peak” in (A,B) are indicated for both I_OGD (n = 23) and V_OGD (n = 12; P = 0.88)). (D) The time to peak (Ctr, n = 23 and TTX, n = 7; P = 0.86, right) and the electrical charge underlying I_OGD (Ctr, n = 19 and TTX, n = 8; P = 0.93, left) are reported in control and in the presence of TTX (1 μM) for each recorded cells: there is no statistical difference between the two cell populations.