FIGURE 2.

Determination of the oligomerization state of the relaxosome proteins Aux1_LS20 and Aux2_LS20. Purified proteins in solution at 12 μM were studied by sedimentation velocity (SV) and sedimentation equilibrium (SE). Plots (A,C) are representations of the sedimentation coefficient distribution c(s) profiles obtained by SV and correspond to Aux1_LS20 and Aux2_LS20, respectively. Concentration gradients obtained by SE assays: (B) Data obtained with Aux1_LS20 (empty circles) and best-fit analysis assuming a protein tetramer (black line), dimer (dashed line) and hexamer (dotted line) species model; (D) Data collected with Aux2_LS20 (empty circles) and best-fitting assuming a protein hexamer (black line), tetramer (dashed line) and octamer (dotted line) species model. Lower part in the SE plots represents the difference between experimental data and estimated values for the best fit to a single species model (residuals).