Table 1.
pLS20cat genes 56 and 57 are required for conjugation.
| Strain | Genotype | Plasmid | Inductor | Conjugation efficiency∗ |
|---|---|---|---|---|
| PKS11 | 168 | pLS20cat | - | 7.9 × 10-3 |
| GR149 | 168 | pLS20Δ56-58 | - | <10-7 |
| GR150 | 168, amyE::Pspank 56-58 | pLS20Δ56-58 | - | <10-7 |
| + | 3.2 × 10-3 | |||
| GR153 | 168, amyE::Pspank 58 | pLS20Δ56-58 | - | <10-7 |
| + | <10-7 | |||
| GR197 | 168, amyE::Pspank 57-58 | pLS20Δ56-58 | - | <10-7 |
| + | <10-7 | |||
| GR200 | 168, amyE::Pspank 58, lacA::Pxyl-56 | pLS20Δ56-58 | - | <10-7 |
| + | <10-7 | |||
| GR225 | 168, amyE::Pspank 57-58, lacA::Pxyl-56 | pLS20Δ56-58 | - | <10-7 |
| + | 5.8 × 10-4 |
∗Conjugation efficiencies were calculated as transconjugants/donor, and correspond to the mean value of at least three independent experiments. When indicated, the inducer IPTG was added at a final concentration of 1 mM in the case of strains GR150 and GR197. In the case of GR200 and GR225, the final concentrations of the inducers was 1 mM (IPTG) and 1% (xylose).