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. 2017 Nov 3;8:1850. doi: 10.3389/fpls.2017.01850

FIGURE 1.

FIGURE 1

Plasmids used to make the auxotrophic PpHIS3Δ and PpTRP1Δ knockout strains and to transform the PpHIS3Δ strain. (A) The knockout plasmid pBHRF-HIS3-5′-3′ used to generate the PpHIS3Δ knockout strain and knockout plasmid pBHRF-TRP1-5′-3′ used to generate the PpTRP1Δ knockout strain. (B) Plasmid pHGZ404 used to transform the PpHIS3Δ knockout strain. Both circular (supercoiled) and linearized pHGZ404 was used to transform the moss. The linearized plasmid comprising two fragments is shown in the figure.