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Assay for G418 resistance in different transformants of the PpHIS3Δ strain with plasmid pHGZ404. Eight linearized (L17–L58) and eight circular (C1–C8) pHGZ404 transformants were grown on G418-containing plates to established to what extent they retained the nptII drug resistance marker. Also shown as controls are the drug sensitive Wt and the stably drug resistant P5 strain, which carries chromosomally integrated copies of the nptII marker at the PpHXK1 locus.
