FIGURE 13.

Plasmid loss experiment to check for retention of G418 resistance in different transformants of the PpHIS3Δ strain with plasmid pHGZ404. Representative photographs of eight linearized (L17–L58) and eight circular (C1–C8) pHGZ404 transformants after growth on G418-containing plates are shown. Colonies that survived due to retention of the episomally replicating nptII marker are green, and those that lost the marker and died are white. Also shown as controls are the drug sensitive Wt and the stably drug resistant P5 strain, which carries chromosomally integrated copies of the nptII marker at the PpHXK1 locus.