Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Polymerase chain reaction verification of the PpHIS3Δ and PpTRP1Δ knockouts. PCRs on genomic DNA from the PpHIS3Δ, PpTRP1Δ, and wild type (Wt) moss strains. PCRs amplifying the 5′-end region (top) and the 3′-end region (middle) are shown, in which one primer was located inside the knockout construct the other primer outside. Also shown is PCRs using two internal primers from the targeted locus (bottom) which verify that the targeted gene has been deleted in the knockout strains.
