Figure 4.

Osthole treatment enhanced the generation of IL-10-producing DCs and Treg cells. (a–g) Frequencies of IL-10-producing cells in the lung or TLNs. Lungs were digested with collagenase A, and single-cell suspensions from lungs or TLNs were acquired as described in ‘Materials and Methods’ section. Cell phenotypes determined by staining as follows: CD11b⁺CD11c⁺ (DCs), CD4⁺Foxp3⁺ (Treg cells), CD4⁺IL-4⁺ (Th2 cells), CCR3⁺Siglec F⁺ (eosinophils), CD117⁺FcεRI⁺ (mast cells) and F4/80⁺CD68⁺ (macrophages). Data are representative dot plots of digested cells with IL-10. Percentages in the top right quadrants refer to percentages of cells expressing IL-10. Values are expressed as the mean±s.e.m. of four mice per group. (h) Data are expressed as cell types producing IL-10 in the median range. *P<0.05 vs the PC group.