Supplement-Table 3. Real-time PCR mix for samples.
V= 25μL | ||
---|---|---|
1. | 10Хbuffer (for Taq- polymerase with SybrGreen)) | 2.5 μL |
2. | 2.5 mM dNTPs | 2.5 μL |
3. | 25 mM MgCl2 | 2.5 μL |
4. | 10 μM Primers | per1.0 μL |
6. | cDNA | 5 μL |
7. | Н2ОQ | 10 μL |
8. | 5000 u/ml Taq_ polymerase | 0.25 μL |