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. 2017 Sep 21;11:77–93. doi: 10.2174/1874091X01711010077

Supplement-Table 3. Real-time PCR mix for samples.

V= 25μL
1. 10Хbuffer (for Taq- polymerase with SybrGreen)) 2.5 μL
2. 2.5 mM dNTPs 2.5 μL
3. 25 mM MgCl2 2.5 μL
4. 10 μM Primers per1.0 μL
6. cDNA 5 μL
7. Н2ОQ 10 μL
8. 5000 u/ml Taq_ polymerase 0.25 μL