Table 2.

Primer sequences for quantitative RT–PCR, ChIP‐PCR, and genotyping in the present study

qRT–PCR
Mouse	Forward	Reverse
Il1β	AGGATGAGGACATGAGCAC	ACGTCACACACCAGCAGGTTATC
Tnfα	ACGCTCTTCTGTCTACTGAACTTC	TGAGGGTCTGGGCCATAGAAC
Il6	AGGCTTAATTACACATGTTCTCTG	TCATCGTTGTTCATACAATCAG
Nfe2l2	AGACACCAGTGGATCCGCC	TACAAATGGGAATGTCTCTGC
Sod1	ATACTGATGGACGTGGAACC	AACCATCCACTTCGAGCAG
Hmox1	ACAGGGTGACAGAAGA	ACTCTGGTCTTTGTGT
Bmi1	TTTATGCAGCTCACCCGTC	CTCCTCATCTGCAACTTCTCC
C/ebpβ	AAGAAGACGGTGGACAAGCTG	TGCTCCACCTTCTTCTGCAGC
Gapdh	TGGAGTCTACTGGTGTCTTC	TCTCGTGGTTCACACCCATC

Human	Forward	Reverse
NFE2L2	AGCACATCCAGTCAGAAACC	TCATCAAAGTACAAAGCATCTG
SOD1	TGGGCCAAAGGATGAA	TGATGCAATGGTCTCC
HMOX1	AGTTCATGAGGAACTTTCAG	TCTCCTTGTTGCGCTCAATCTC
C/EBPβ	AGAAGGTGGAGCAGCTGTC	AGCTGCTTGAACAAGTTCC
GAPDH	AAGTATGACAACAGCCTCAAG	TCCACGATACCAAAGTTGTC

ChIP‐PCR
Mouse	Forward	Reverse
Nfe2l2 promoter	ATTCTGCCTCAATTTTCTTAGC	ATCCAATTCCGACTCTTTTG
Sod1 promoter	ACTTTCACACTGCAACCG	TCGCTATTGTCTTCTAACTGTG
Hmox1 promoter	ACCAAGCAGAACTGTGAAC	AGAATTTGGGTCCTTTCC
Positive control (Bmi1 promoter)	ACTACACCGACACTAATTCCCAG	TCCAAAATGGCTCGGAGTCC
Negative control (Patl2 intron)	TCAGGTCCTAAGTCCTTTTCTGAGTGG	TGGACCCTTGGTGCCCTACTATCTAG

Genotyping	Forward	Reverse
miR‐155 Knockout	TCTGACATCTACGTTCATCC	ACTAACTGTGTGCGTACACAC