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. 2017 Oct 5;16(6):1404–1413. doi: 10.1111/acel.12682

Figure 4.

Figure 4

mtDNA depletion and energy failure in Sirt2 −/− mice. (A) SIRT2 protein level in spinal cord from WT and Sirt2 −/− mice at 13 months of age (n = 6/genotype), (B) mtDNA levels in spinal cord and cortex (n = 8/genotype), (C) relative mRNA levels of Sirt2, Sirt1, Pgc‐1α, Tfam, and Nrf1 in spinal cord and cortex (n = 8/genotype), (D) SIRT1 protein level in spinal cord (n = 6/genotype), (E) ATP levels in spinal cord and cortex (n = 8/genotype), (F) ex vivo mitochondrial respiration analysis performed on permeabilized sections of spinal cord (n = 5/genotype), and (G) NAD +, NADH, and NAD +/NADH ratio in spinal cord and cortex (n = 8/genotype) from WT and Sirt2 −/− mice at 13 months of age. Data represent mean ± SD. Statistical analysis was carried out with Student's t‐test (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001).