Fig. 2.

Effect of miR-195 on the protein expression of PEPCK. HepG2 cells were reverse-transfected with scRNA (200 nM) or miR-195 (200 nM). After 48 h transfection, the protein expression of PEPCK was analyzed by immunoblotting. (A) Representative immunoblot obtained from three independent experiments were shown. (B) The immunoblot intensities for PEPCK/β-Actin were quantified by densitometry and expressed in relative ratio. The intensity of basal scRNA control was set to one. Values are expressed as means ± SEM of three independent experiments. ***, P < 0.001 vs. basal scRNA control; ###, p < 0.001 scRNA + Insulin vs. miR-195 + Insulin.