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. 2017 Sep 7;207(3):1041–1051. doi: 10.1534/genetics.117.300260

Figure 2.

Figure 2

oko meduzy and crb3a genes modulate cilia length. (A–O) Whole-mount immunostaining of wild-type (WT), ome−/−, and ome−/−;3a−/− double mutant cilia in several tissues at 5 days postfertilization. (A–C) lateral crista, (D–F) anterior macula, (G–I) posterior macula, (J–L) pronephros, and (M–O) nasal pits. Zebrafish larvae were immunostained using anti-acetylated tubulin antibody (in green) and counterstained with DAPI (in blue) to visualize nuclei. (P) Graph of cilia length in the cristae of WT and crumbs mutants as indicated. Each dot represents the average length of all cilia in one crista. (Q) Graph of cilia length in the olfactory placodes of WT and crumbs mutants as indicated. In (P and Q), data were collected from at least two independent experiments using at least five animals per experiment. The mean and 95% C.I. are indicated. Based on Student’s t-tests; ** P < 0.01, *** P < 0.001, and **** P < 0.0001; not significant, ns. All differences were also significant based on Mann–Whitney test.