Figure 5.

Intraflagellar transport (IFT) particle components accumulate in the ciliary compartment of crumbs mutants. Confocal images of whole-mount cilia staining with antibodies to acetylated tubulin (AcTub) (green), and to IFT proteins (in red): IFT88, IFT52, and Kif17 at 5 days postfertilization. Shown are cristae and olfactory placodes of wild-type (WT), ome−/−, crb3a−/−, and ome−/−;crb3a−/− double mutants as indicated. IFT proteins are not detected in the ciliary shaft of WT cristae using this staining method (A–C’) and a low amount of IFT52 is found in WT olfactory cilia (D–D’’). Similarly, in crb3a−/− mutants, IFT proteins are not detected in cilia (E–H’’). Low levels of some IFT proteins are found in cristae cilia of ome−/− mutants (I–K’). Compared to WT, IFT52 localization is not affected in olfactory placode cilia of ome−/− mutants (L–L’’). In contrast to that, IFT proteins, including Kif17, strongly accumulate in cilia of ome−/−;crb3a−/− double mutants (M–P’’). All samples were counterstained with DAPI to visualize nuclei (in blue). Brackets in (D’, H’, L’, and P’) indicate nasal cilia.