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. 2017 Sep 7;207(3):911–922. doi: 10.1534/genetics.117.300259

Figure 5.

Figure 5

Chromosomes lag during anaphase I and anaphase II in 3N embryos. (A) Chromosomes undergo two rounds of separation during anaphase I and anaphase II in a 2N control embryo expressing GFP::Tubulin (TUB) and mCh::His. (B) Anaphase I in a 3N embryo shows a chromosome that lags as it travels to the cortical pole. (C) Anaphase I in a 3N embryo shows two chromosomes which lag as they travel toward the interior pole. One of the lagging chromosomes appears to be a univalent, which separates into two chromatids as anaphase I ends and MII spindle formation begins. (D) Anaphase II in a 3N embryo shows a chromosome that lags as it travels to the cortical pole. (E) Anaphase II in a 3N embryo shows a chromosome that lags as it travels to the interior pole. Lagging chromosomes were observed in 14/15 anaphase I embryos and 17/20 anaphase II embryos from triploid hermaphrodites. Bar, 5 μM.