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. 2017 Sep 20;207(3):1157–1166. doi: 10.1534/genetics.117.300306

Figure 1.

Figure 1

(A) Examples of imaged eyes from various D. melanogaster alleles; (a) w1118, (b) wm4 with the Df(2L)2802 second chromosome, (c) wm4 with the CyO second chromosome, (d) bw1, (e) wm4 with Df(2L)2802/CyO, and (f) Canton-S. (B) Scatter plot of PC1 and PC2 values for mutant and experimental PEV eyes. Black and white points represent individual images and average values of eye colors from stocks; (a) wm4,(d) bw1, (e) wm4 with Df(2L)2802/CyO, (f) Canton-S. Experimental individuals with just the Df(2L)2802 (periwinkle), in general, show greater variegation and eyes that are closer to the w1118 allele (a), an eye that lacks pigmentation. Experimental siblings with the CyO allele (red) show less variegation, or more pigmentation. (C) Boxplot of PEV summarized by line (x-axis) and separated by second chromosome, Df(2L)2802 (periwinkle) vs. CyO (red). PEV is represented PC1 (y-axis), where lower values indicate less pigmented eyes, and higher values indicate more pigmented eyes. Among-line variance is greater than within-line variance, suggesting natural genetic variation is involved in observed differences in PEV.