Figure 4.

Survival of cells that experienced dicentric formation. Dicentric chromosome formation was induced in larvae of all stages by heat shock (38°, 1 hr) using 70FLP3F. P{GawB}6011A drove expression of UAShiphop in the brain. Metaphase nuclei from third instar larval brains were examined at 24 and 72 hr after heat shock. Cells carrying broken chromosomes (visibly shortened) and/or acentric chromosomes were scored as abnormal. The numbers of nuclei examined for each time point and genotype were 115 (5 brains), 99 (4 brains), 133 (4 brains), and 33 (3 brains), respectively. Significance was calculated with a 2 × 2 contingency test. SD are indicated.