Figure 6.

Expression of UAShiphop in the male germline. Testes of y w/Y; UAShiphop/+; nosGal4 UASFLP/+ males were dissected and stained with DAPI and an antibody to mCherry (to which Hiphop is fused in this construct). The HRH008-A1 insertion and the nGUF95 combination were used (the same combination as in Figure 5, third column, second and fourth rows). Seven testes were examined—all showed strong expression in germline stem cells at the hub (circled in panel at right), and variable expression at later stages. Expression was in the form of bright puncta, as expected for telomeric staining, and a diffuse and dimmer cytoplasmic staining.