Table 2. Absorbance Maxima of DTB-SQ Absorption Band Measured with LmrR_M89X and LmrR_M89X_V15E_F93D, in the Presence of Transition-Metal Ions.
M89X |
M89X_V15E_F93D |
|||
---|---|---|---|---|
λmax(1) (nm) | λmax(2) (nm) | λmax(1) (nm) | λmax(2) (nm) | |
Cu(II) | 764 | ∼850 | 765 | ∼850 |
Zn(II) | 739 | 789 | 733 | 801 |
Co(II) | 733 | 780 | 732 | 788 |
Ni(II) | 758 | 813 | 764 | 817 |
Fe(II)a | n.d. | n.d. | 745 | 803 |
Protein incubated with an excess of Fe(II) followed by dialysis to remove the excess of metal ions.