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. 2017 Dec;92(6):630–639. doi: 10.1124/mol.117.109462

Fig. 6.

Fig. 6.

A1R activation led to sustained localization of RGS4 at the plasma membrane of live cells with expression of full-length neurabin but failed to do so when Nrb1-597 was expressed. (A) Representative images of RGS4 localization before and after 1 μM R-PIA stimulation in Cos7 cells coexpressing A1R-CFP and RGS4-YFP with full-length neurabin. (B) Representative traces of RGS4 showing sustained RGS4 translocation within different plasma membrane ROIs in a single Cos7 cell with full-length neurabin coexpressed with A1R-CFP and RGS4-YFP (n = 6). R-PIA (1 μM) was added at t = 0. (C) Representative traces showing transient RGS4 translocation within different plasma membrane ROIs in a single Cos7 cell with Nrb1-597 coexpressed with A1R-CFP and RGS4-YFP (n = 4). R-PIA (1 μM) was added at t = 0. (D) Multiple translocation traces from different cells expressing the full-length neurabin demonstrate uniform responses of sustained RGS4 localization at the plasma membrane across the cells; n = 8. (E) Multiple traces from different cells expressing Nrb1-597 demonstrate nonuniform responses of RGS4 translocation across the cells with a general transient nature (n = 6). (F) The sustained plasma membrane localization of RGS4 was analyzed by comparing the slope of the trace after the maximal response in each cell. ***P < 0.001 between the slopes of cells in the presence of full-length neurabin and Nrb1-597A.