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. 2016 Sep 27;19(6):707–714. doi: 10.4103/1008-682X.185848

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Copyright: © The Author(s)(2017)

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Identification of AMPK protein in human sperm by Western blotting. (a) Human sperm proteins (10 μg) from different donors were analyzed by Western blot using anti-AMPKα1/2 as the primary antibody. (b) Different patterns of AMPK cross-reacted bands in several donors of human sperm. Arrows indicate cross-reactive bands of AMPK protein recognized by the antibody. Molecular weight markers are indicated in KDa. Loading control was performed for each experiment in the same membranes using the anti-α tubulin antibody and is shown on the lower panels. Each experiment was performed seven times and representative films are shown.