Figure 3.

Combined effects of fentanyl and TRV130 on antinociception in mice. Enhancement of the antinociceptive effect produced by fentanyl (60 µg/kg, s.c.) and/or TRV130 (0.5 and 1.0 mg/kg, s.c.) using a hot-plate test. Antinociception is expressed as the % maximum possible effect (MPE). Each column represents the mean ± SEM of six to nine mice. *p < 0.05 and ***p < 0.001 versus saline control (a). Changes in the latency of paw withdrawal in response to a thermal stimulus in mice after sciatic nerve ligation after repeated treatments with fentanyl (30 µg/kg, s.c.) or fentanyl (30 µg/kg, s.c.) plus TRV130 (0.3 mg/kg, s.c.) for seven days. Each column represents the mean ± SEM of 6–10 mice. The dotted line indicates the mean control latency of paw withdrawal in sciatic nerve-ligated mice. *p < 0.05 versus sham-saline-saline group. #p < 0.05 versus ligation-fentanyl-TRV130 group.