Figure 1.

Effect of intrathecal administration of decitabine (Dec) on the development of mechanical allodynia, thermal hyperalgesia, and cold allodynia induced by injecting prostate cancer cells (PCC) into the tibia. Decitabine given once daily for seven days dose dependently blocked PCC-induced decreases in paw withdrawal threshold (PWT) to mechanical stimulation (a) and paw withdrawal latency (PWL) to thermal (b) or cold (c) stimulation on the ipsilateral side. Decitabine at 20 µg alone did not affect basal responses to mechanical, thermal, or cold stimuli on the ipsilateral side in rats injected with Hank’s balance salt solution (HSBB, (a) to (c)). Decitabine at the doses used did not affect basal responses to mechanical (d) and thermal (e) stimuli on the contralateral side. n = 5/group. Two-way ANOVA followed by post hoc Tukey’s test. *P < 0.05, **P < 0.01 versus the vehicle plus PCC group at the corresponding time points.