Table 1.

Gene-specific primers were used to amplify the target mRNA of cytokines and T-cell makers by RT-qPCR.

S. No	Primer name	Sequence (5′—>3′)
1	GM-CSF	FP: 5′-GCCATCAAAGAAGCCCTGAA-3′RP: 5′-GCGGGTCTGCACACATGTTA-3′
2	IL-10	Forward 5′-GGTTGCCAAGCCTTATCGGA-3′Reverse 5′-ACCTGCTCCACTGCCTTGCT-3′
3	IL-12p40	Forward 5′-GGAAGCACGGCAGCAGAATA-3′Reverse 5′-AACTTGAGGGAGAAGTAGGAATCG-3′
4	IFN-γ	Forward 5′-TCAAGTGGCATAGATGTGGAAGAA-3′Reverse 5′-TGGCTCTGCAGGATTTTCATG-3′
5	IL-4	Forward 5′-ACAGGAGAAGGGACGCCAT-3′Reverse 5′-GAAGCCCTACAGACGAGCTCA-3′
6	TGF-β	Forward 5′-TGACGTCACTGGAGTTGTACGG-3′Reverse 5′-GGTTCATGTCATGGATGGTGC-3′
7	GAPDH	Forward 5′-CAAGGCTGTGGGCAAGGTCA-3′Reverse 5′-AGGTGGAAGAGTGGGAGTTGCTG-3′
8	Granzyme-A	FP: 5′-CAT TGG AGG AGA CAC GGT TGT TCC-3′RP: 5′-CTC TTT CCC ACG TTA CAG TGG GC-3
9	CTLA-4	FP: 5′-GGACTTGGCCTTTTGTAGCCCT-3′RP: 5′-ATT CAC ATG GAA AGC TGG CGA CAC-3′
10	PD-1	FP: 5′-CCTGGTCATTCACTTGGGCTGTG-3′RP: 5′-GGT GGC ATT TGC TCC CTC TGA-3′