Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 7;7:14634. doi: 10.1038/s41598-017-12660-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

miR-92b-3p secretion with tumour-derived exosomes. (A) Scanning electron microscope image of purified exosomes, which were essentially homogeneous at 40–200 nm in diameter. Bar: 100 nm. (B) The size distribution of exosomes assessed by the NanoSight^® nanoparticle tracking system. The size range of isolated exosomes was approximately 50–200 nm, peaking at 100 nm. (C) Western blotting of the exosomes derived from SS (SYO-1, HS-SY-II, YaFuSS). Staining for tetraspanin protein (CD9, 25 kDa) was positive for both cell lysates and exosomes, whereas probing for cytochrome-c (15 kDa) was negative for exosomes. Full-length blots are presented in Supplementary Figure S2. (D) Polymerase chain reaction of SS18-SSX fusion gene. SS18-SSX was detected for both cells and exosomes of cultured SS cells (SYO-1, HS-SY-II, YaFuSS). HT1080 fibrosarcoma cell line was used as negative control. Full-length gels are presented in Supplementary Figure S3. (E) miR-92b-3p expression levels in SS-derived exosomes. hMSCs was used as a negative control *p < 0.05; Student’s t test. (F) Western blotting of SS patient serum for each fraction of EV-second^® procedure. Fraction 3 to 7 were positive for CD9 (25 kDa). Strongly positive fractions 4 to 6 mainly contain exosomes. Full-length blots are presented in Supplementary Figure S4. (G) Fractions containing exosomes (fractions 4 to 6) and subsequent fractions (fractions 9 to 11), which contain larger-sized proteins than earlier fractions, which were immunoprecipitated using human anti-Ago2 monoclonal antibody. SYO-1 was used as a positive control. Western blotting revealed Ago2 (100 kDa) was negative in exosome fractions and positive for fractions 9 to 11, suggesting these fractions mainly contain Ago2. Full-length blots are presented in Supplementary Figure S5. (H) The expression of miR-92b-3p in exosomes and Ago2 concentrations *p < 0.05; Mann-Whitney U test.