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TLS in high grade MIBC. H & E stained 5 μm thick FFPE section of MIBC tumour showing TLS like lymphoid aggregation (A, C and E) further confirmed by staining with multi-color IHC (B, D and F) markers specific to TLS including, CD3+ (green) and CD8+ T cells (purple), CD20+ B cells (pink), CD21+ follicular dendritic cells (blue), CD208+ mature dendritic cells (black), PNAd+ high endothelial venule (brown).
