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. 2017 Nov 7;8:1356. doi: 10.1038/s41467-017-01291-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Assessment of different variations of TeSLA. a TeSLA of human bronchial epithelial cells (HBECs), Calu 6 (lung cancer cell line), and mixed DNA (HBEC: Calu 6 = 1:1) using 30 pg of DNA for each TeSLA PCR. b The kernel density estimation of TL from TeSLA results of Calu 6 (blue), HBEC (orange), and mixed DNA (red). The reference line (green dashed) represents a theoretical density function of TeSLA results when HBEC: Calu 6 = 1:1. c Standard deviations of bootstrapped distributions of mean TLs computed from 32 TeSLA PCRs of HBECs (Supplementary Fig. 4a) show the estimation accuracy achieved by using n (1 ≤ n ≤ 32) lanes to estimate the mean TL. d Coverage rates estimated from 32 TeSLA PCRs of HBECs (Supplementary Fig. 4a) based on bootstrapping. When eight PCRs were randomly selected from the 32 PCRs, 87% of all telomeres were detected with bin sizes 0.5 kb ranging from 0 to 10 kb. Red (yellow) line indicates upper (lower) 95% confidence bounds of the coverage rates. e Empirical distribution curves of quadruplets (eight TeSLA PCRs of each) from TeSLA results for HBECs show no significant changes in each eight TeSLA PCRs. f Representative TeSLA results of PBMCs from a young (age 32) and an old (age 72) individual. g Empirical distribution curves of TLs from the TeSLA of the triplicate results (blue, red, and yellow lines) for the 32-year-old male and triplicate results for the 72-year-old male (purple, green, and sky blue lines)