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. 2017 Nov 7;8:1356. doi: 10.1038/s41467-017-01291-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — TeSLA is sensitive enough to detect changes of TLs in a 1-year period of normal human aging. a, b Scatter dot blots comparing TLs in PBMCs measured at baseline and in 1-year period by TeSLA (a) and TRF analysis (b). The mean, the median, and the shortest 20% TLs of 15 normal healthy subjects (age from 51 to 69) were averaged. P-values from paired t-tests are shown as indicated above. BL, baseline; 1 yr, one year after; NS, not significant. c, d The average changes of TL distributions in PBMCs in a 1-year period of 15 subjects measured by TeSLA (c) and TRF analysis (d). One-year differences in cumulative frequencies from each subject were computed (see Supplementary Fig. 5e, f as examples). The average of 1-year changes in TL distributions of 15 subjects is shown in red, and one-sided 95% confidence limit (black) is derived from permutation. The asterisk represents the value (~1 kb of TL) that lies outside the 95% confidence limit, which indicates the most significant effect on telomere shortening. e, f Scatter plots comparing TeSLA and TRF analysis for mean (e) and median (f) TL measurements (n = 30) in PBMCs. g Comparison of TeSLA and TRF analysis of empirical distribution curves of pooled TLs from all 30 DNA samples. h The averaged differences (red) in cumulative frequencies (TeSLA-TRF) by the same method used in c and d show large difference between TeSLA and TRF in the short TL analyses (0.6–2.8 kb). Black lines are 95% confidence limits obtained from permutation. (a, b; mean and s.e.m., n = 15) (e, f; mean and s.e.m., n = 30)