Table 2.
The co-localization pattern of CB+ neurons with ERα, ERβ and AR in the amygdala of the guinea pig in male and female subjects
Calbindin | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
ERα | ERβ | AR | |||||||||
CB+ | CB+/ERα+ | % | CB+ | CB+/ERβ+ | % | CB+ | CB+/AR | % | |||
LA | |||||||||||
♂ | 527.3 ± 33.7 | 4.8 ± 0.3 | 0.91 ± 0.07 | 564.2 ± 26.1 | 34.6 ± 2.4 | 6.13 ± 0.45 | 571.8 ± 43.1 | 0 | – | ||
♀ | 560.9 ± 42.8 | 4.1 ± 0.4 | 0.73 ± 0.05 | 587.4 ± 47.9 | 31.3 ± 2.9 | 5.33 ± 0.31* | 601.6 ± 31.7 | 0 | – | ||
BL | |||||||||||
♂ | 593.5 ± 31.9 | 0 | – | 549.6 ± 28.6 | 18.5 ± 2.1 | 3.36 ± 0.22 | 535.8 ± 23.6 | 0 | – | ||
♀ | 624.1 ± 50.3 | 0 | – | 605.5 ± 33.9* | 24.3 ± 1.7** | 4.01 ± 0.39* | 574.1 ± 38.3 | 0 | – | ||
BM | |||||||||||
♂ | 379.1 ± 17.4 | 3.2 ± 0.3 | 0.84 ± 0.05 | 432.3 ± 32.8 | 61.8 ± 3.6 | 14.29 ± 0.59 | 411.2 ± 27.3 | 23.5 ± 1.1 | 5.73 ± 0.55 | ||
♀ | 415.5 ± 25.6 | 3.8 ± 0.2 | 0.90 ± 0.07 | 488.8 ± 29.5* | 65.6 ± 4.3 | 13.43 ± 0.91 | 457.6 ± 35.6* | 22.6 ± 1.4 | 4.94 ± 0.34 | ||
CE | |||||||||||
♂ | 317.8 ± 15.9 | 0 | – | 292.8 ± 15.7 | 3.1 ± 0.4 | 1.05 ± 0.11 | 273.7 ± 20.2 | 17.2 ± 1.2 | 6.28 ± 0.46 | ||
♀ | 308.3 ± 22.1 | 0 | – | 303.1 ± 19.3 | 2.6 ± 0.3 | 0.86 ± 0.09* | 267.3 ± 15.7 | 19.7 ± 1.5* | 7.11 ± 0.58 | ||
ME | |||||||||||
♂ | 618.1 ± 38.2 | 3.1 ± 0.5 | 0.51 ± 0.07 | 650.4 ± 39.2 | 4.4 ± 0.3 | 0.68 ± 0.08 | 664.6 ± 29.1 | 241.5 ± 19.3 | 36.34 ± 2.45 | ||
♀ | 549.8 ± 26.3 | 3.8 ± 0.4 | 0.69 ± 0.04 | 581.9 ± 27.8* | 5.1 ± 0.4* | 0.87 ± 0.11* | 603.2 ± 40.2* | 209.1 ± 17.1* | 34.65 ± 1.89 | ||
CO | |||||||||||
♂ | 475.7 ± 57.9 | 3.5 ± 0.4 | 0.72 ± 0.04 | 508.6 ± 24.1 | 56.2 ± 4.3 | 11.04 ± 0.93 | 518.9 ± 34.3 | 24.4 ± 2.1 | 4.71 ± 0.39 | ||
♀ | 469.7 ± 42.6 | 2.2 ± 0.1 | 0.47 ± 0.06 | 491.9 ± 41.2 | 47.9 ± 3.8* | 9.73 ± 0.71 | 487.3 ± 27.6 | 17.3 ± 1.3** | 3.56 ± 0.43** |
Note in both sexes modest co-localization of CB+ neurons with ERβ and AR as well as the lack of associations of these cells with ERα. Data were expressed as mean ± standard deviation (SD). As CB+ neurons almost never co-express ERα, only the counts concerning relationships of CB+ neurons with ERβ or AR were statistically evaluated by the two-tailed t test (* p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001)