Table 5.
The co-localization pattern of CR+ neurons with ERα, ERβ and AR in the amygdala of the guinea pig in male and female subjects
| Calretinin | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ERα | ERβ | AR | |||||||
| CR+ | CR+/ERα+ | % | CR+ | CR+/ERβ+ | % | CR+ | CR+/AR | % | |
| LA | |||||||||
| ♂ | 1281.6 ± 68.8 | 238.4 ± 18.2 | 18.6 ± 0.96 | 1309.1 ± 96.7 | 0 | – | 1231.0 ± 76.3 | 0 | – |
| ♀ | 1317.5 ± 93.3 | 280.6 ± 24.7* | 21.3 ± 1.45* | 1328.4 ± 81.9 | 0 | – | 1198.3 ± 109.8 | 0 | – |
| BL | |||||||||
| ♂ | 440.9 ± 18.6 | 27.8 ± 2.3 | 6.3 ± 0.84 | 461.5 ± 23.0 | 0 | – | 429.4 ± 29.9 | 3.4 ± 0.4 | 0.8 ± 0.06 |
| ♀ | 459.4 ± 26.5 | 21.1 ± 2.8* | 4.6 ± 0.69* | 470.2 ± 27.2 | 0 | – | 442.2 ± 25 | 3.1 ± 0.3 | 0.7 ± 0.11 |
| BM | |||||||||
| ♂ | 357.7 ± 27.1 | 120.5 ± 9.1 | 33.7 ± 1.97 | 317.1 ± 17.7 | 3.5 ± 0.3 | 1.1 ± 0.14 | 334.3 ± 26.1 | 25.7 ± 1.8 | 7.7 ± 0.63 |
| ♀ | 369.2 ± 18.4 | 129.9 ± 8.2 | 35.2 ± 1.81 | 335.2 ± 24.3 | 2.7 ± 0.2 | 0.8 ± 0.09 | 350.3 ± 21.5 | 23.8 ± 1.7 | 6.8 ± 0.41 |
| CE | |||||||||
| ♂ | 12.3 ± 1.0 | 0 | – | 8.6 ± 0.9 | 0 | – | 11.2 ± 0.9 | 0 | – |
| ♀ | 13.6 ± 0.8 | 0 | – | 12.7 ± 1.1 | 0 | – | 12.7 ± 0.7* | 0 | – |
| ME | |||||||||
| ♂ | 422.6 ± 25.2 | 127.2 ± 6.6 | 30.1 ± 1.24 | 362.3 ± 23.9 | 2.8 ± 0.1 | 0.8 ± 0.05 | 394.9 ± 31.6 | 70.3 ± 5.1 | 17.8 ± 1.13 |
| ♀ | 403.8 ± 37.1 | 112.7 ± 8.6* | 27.9 ± 2.01 | 349.0 ± 14.5 | 1.7 ± 0.1 | 0.4 ± 0.03 | 377.6 ± 28.1 | 57.8 ± 4.2** | 15.3 ± 0.85* |
| CO | |||||||||
| ♂ | 386.4 ± 23.9 | 90.4 ± 3.9 | 23.4 ± 1.76 | 396.4 ± 28.4 | 0 | – | 361.3 ± 19.2 | 13.0 ± 0.6 | 3.6 ± 0.28 |
| ♀ | 370.7 ± 17.8 | 84.5 ± 5.8 | 22.8 ± 1.36 | 407.6 ± 21.5 | 0 | – | 339.7 ± 25.1 | 13.9 ± 0.8 | 4.1 ± 0.37 |
Note that in both sexes CR+ neurons show extensive co-localization with ERα and modest co-expression with AR, however, these cells almost never co-express ERβ. Data were expressed as a mean ± standard deviation (SD). As CR+ neurons almost never co-express ERβ, only the counts concerning relationships of CR+ neuron with ERα or AR were statistically evaluated by the two-tailed t test (* p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001)