Fig. 4.

Reduced number of interneurons and pyramidal cells in the cortex of Cdh13 ^−/− mice. Images of in situ hybridization for GAD67 (a, b) and immunohistochemistry for Ctip2 (e, f), Cux1 (i, j) and CC3 (m, n) in coronal sections through the cortex of Cdh13 ^+/+ (a, e, i, m) and Cdh13 ^−/− (b, f, j, n) mice at E18.5. Analysis of the number of GAD67 labelled cells in all layers of the cortex at E15.5 (c) and E18.5 (g) as well as their relative distribution compared to wild-type litter mates at both ages (d, h). k Quantification of Ctip2 and Cux1⁺ cells in the cortex of Cdh13 ^−/− animals showed reduced numbers of the latter only at E18.5 when compared to Cdh13 ^+/+ controls. l Quantification of the number of CC3+ cells in the CP (black arrows in m, n) showed an increase in the cortex of Cdh13 ^−/− mice compared to Cdh13 ^+/+ controls at E18.5 [CC3+ blood vessels (white arrows in m, n, were excluded from counts)]. o Reduced cortical thickness was observed in the cortex of Cdh13 ^−/− animals at E18.5 when compared to Cdh13 ^+/+ controls, to coincide with the decreased number of neurons. Scale bars in a 200 µm, in e 100 µm and in m 150 µm. (*p < 0.01, ***p < 0.0001). Error bars indicate SEM. CP cortical plate, IZ intermediate zone, LCP lower CP, MZ marginal zone, SVZ subventricular zone, UCP upper CP, VZ ventricular zone