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Secreted EVs can be successfully isolated from neuroglial co-cultures. Transmission electron microscopy confirming the presence of an EV (asterisk) in close proximity to multivesicular astrocytes (A). The EV preparation included smaller vesicles, such as exosomes (B) and larger MVs (C). The size of the exosomes ranged from 50–75 nm and the larger MVs were up to 400 nm in diameter. Scale bars: A = 1μm, B-C = 100 nm.
