Fig.5.

Proteins with altered levels in EVs following Aβ₄₂ protofibril exposure. Mass spectrometry analysis on the protein content in EV lysates revealed 807 unique proteins in total (q-value < 0.05). After further statistical testing and strict quality control, five proteins were identified to be significantly differentially expressed in EVs from Aβ₄₂ protofibril exposed cultures, compared to unexposed cultures for any of the two time points (day 0–2 and day 2–5). The only protein that showed significantly altered levels and at both time points was apoE. Data is presented as relative fold change of apoE in Aβ₄₂ protofibril exposed versus unexposed cultures and was performed in three independent cell culture experiments.