Fig.7.

Apolipoprotein E is primarily situated inside isolated EVs. Immunoprecipitation was performed to confirm the presence of apoE inside the EVs. Following ultracentrifugation, EVs were either resuspended in PBS (intact EVs) or lysis buffer with protease inhibitors (lysed EVs) and immunoprecipitated with apoE antibodies. Western blot analysis from day 0–2 revealed twice as much apoE in the lysed EV samples, compared to the samples with intact EVs (A). The mean apoE intensity was 7.2E5±1.1E5 and 16.3E5±1.2E5 for intact EVs and lysed EVs, respectively (B). The data is from three independent cell culture experiments.