Fig.9.

Intracellular apoE levels are similar in Aβ₄₂ protofibril exposed cells and unexposed cells. Western blot analysis of cell lysates from day 2 verified that there was no difference in intracellular apoE expression in Aβ₄₂ protofibril exposed cultures and unexposed cultures (A,B). The mean apoE intensity was 38.9E5±18.9E5 in unexposed samples and 37.0E5±11.9E5 in Aβ₄₂ protofibril exposed samples. Flotillin-1 levels also remained unchanged in Aβ₄₂ protofibril exposed cells, compared to untreated cells (C,D). The mean flotillin-1 intensity was 22.2E5±5.3E5 in unexposed samples and 22.7E5±5.8E5 in Aβ₄₂ protofibril exposed samples. Six independent cell culture experiments were analyzed for each of the two proteins and the data was examined with Mann-Whitney U-test. Western blot analysis from three independent experiments are illustrated in A and C.