Fig. 4.

Bag6 mutations at the TRC35 binding site results in nuclear localization of Bag6. (A–F) Representative images of Cos7 cells expressing WT TRC35⋅FLAG and WT or mutant Bag6⋅GFP. Cos7 cells were transfected either with Bag6⋅GFP (WT or mutant) expressing plasmid alone or cotransfected with TRC35•FLAG (WT) expressing plasmid. Cells were stained with anti-GFP (green) and/or anti-FLAG (red) Abs. DNA was stained with DAPI where indicated (blue). In the Bag6 column, the percent of cells that contain nuclear Bag6 is noted in parenthesis. For each Bag6 variant, ∼100 cells were counted and categorized as having nuclear or cytosolic Bag6.