Fig. S3.

Representative aligned sequences of eukaryotic TRC35/Get4. Species selected based on the eukaryotic phylogenetic tree by Eme et al. (51). Sequences were aligned with MAFFT (57). α-Helices, based on the TRC35 structure, are highlighted in colors that correspond to the crystal structure on Fig. 1B and numbered on top. Residues highlighted in red boxes were identified as critical to fungal Get4 binding Get3 (13). Residues highlighted in blue boxes are critical to Get4/TRC35 regulating Get3/TRC40 (4, 13). The residues that comprise the fungal β-hairpin are highlighted in a black box. The arrow indicates the end of the crystallization construct. Purple boxes highlight glutamate and aspartate residues in the C terminus of TRC35.