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The effect of RNF126 on TRC35 stabilization. Immunoblot analysis of the effect of RNF126 on TRC35 levels in wt293T cells cotransfected with plasmids encoding TRC35⋅FLAG (WT), Bag6⋅GFP (WT or mutants), and HA⋅ubiquitin. A combination of three siRNAs against RNF126 was used. Bag6 and TRC35 levels were assessed by immunoblotting with anti-GFP and anti-FLAG Abs.
