Table S1.
Data collection and refinement statistics
| Statistics | Native | Rubidium |
| Data collection | ||
| Protein | Bag6–TRC35 | Bag6–TRC35 |
| Synchrotron | SSRL | SSRL |
| Beamline | 12-2 | 12-2 |
| Space group | P 21 21 21 | P21 21 21 |
| Cell dimensions | ||
| a, b, c, Å | 41.7, 84.6, 102.6 | 42.0, 84.3, 102.5 |
| α, β, γ, ° | 90.0 90.0, 90.0 | 90.0, 90.0, 90.0 |
| Wavelength | 0.9200 | 0.8154 |
| Resolution, Å | 39.1–1.80 (1.92–1.80) | 50.0–1.99 (2.11–1.99) |
| Rmeas*, % | 9.3 (64.3) | 16.9 (118.8) |
| I/σ(I) | 16.9 (3.8) | 14.9 (2.1) |
| CC1/2, % | 99.9 (95) | 99.9 (88.0) |
| Completeness*, % | 98.7 (92.7) | 99.2 (95.6) |
| No. of observations | 384,047 (60,190) | 655,714 (96,962) |
| No. of unique reflections* | 40,776 (6,194) | 47,793 (7,417) |
| Redundancy* | 9.4 (9.7) | 13.7 (13.1) |
| Refinement | ||
| Resolution, Å | 39.1–1.80 | |
| No. of reflections | 34,334 | |
| No. of reflections test set | 1,998 | |
| Rwork/Rfree | 15.8/20.0 | |
| No. atoms (nonhydrogen) | 2,738 | |
| Protein | 2,574 | |
| Water | 152 | |
| Ligand/Ions | 12 | |
| B-factors | ||
| Protein | 31.7 | |
| Water | 39.0 | |
| RMSD | ||
| Bond lengths, Å | 0.010 | |
| Bond angles, ° | 1.16 | |
| Ramachandran plot | ||
| Favored, % | 98 | |
| Additionally allowed, % | 2.0 | |
| Outliers, % | 0.0 |
*
Highest-resolution shell is shown in parentheses.