Fig. S5.

Percentage of CCL5 receptors expressed on NK cells infiltrated in Ctrl and BECN1⁻ B16-F10 tumors. NK cells infiltrating Ctrl and BECN1⁻ B16-F10 tumors were stained with NK1.1-, CCR1-, CCR3-, and CCR5-specific antibodies and subjected to flow cytometric analyses. NK1.1⁺/CD45⁺CD11b⁻CD19⁻ cells were gated and the percentage of CCR1⁺, CCR3⁺, and CCR5⁺ NK cells were determined. (A) Representative flow cytometry plots of NK1.1⁺CCR1⁺, NK1.1⁺CCR3⁺, and NK1.1⁺CCR5⁺ in Ctrl and BECN1⁻ B16-F10 tumors. Quadrant settings were based on the cells stained with FMO and then applied to the plots of cells stained with positive antibodies. (B) Percentage of CCR1⁺, CCR3⁺, and CCR5⁺ total NK cells infiltrating Ctrl and BECN1⁻ tumors. Not-significant differences were observed in CCR1, CCR3, and CCR5 between Ctrl and BECN1⁻ tumors.