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The high phosphorylation level of c-Jun on Ser-63 in BECN1− B16-F10 cells is not affected by silencing CCL5. Control and BECN1− B16-F10 cells displaying a high level of Ser-63 phosphorylated c-Jun were transfected with shRNA targeting CCL5. The expression of Beclin1 and Ser-63 (pS63) c-Jun was assessed by Western blot. Actin was used as a loading control.
