Fig. S2.
The effects of different fermentation strategies on (A) ethanol yields (percentage of theoretical maximum) and (B) cell viability in terms of specific colony-forming units (colony-forming units per milliliter) for SSF of pressed CELF-pretreated solids. The final solids loading was 20% and cellulase loading was 5 mg protein⋅g glucan−1. Feeding strategies 1, 2, and 3 refer to the strategies outlined in Table S1. The 2× inoculum strategy refers to the doubling of the initial inoculum during inoculation. Finally, the 18-h prehydrolysis strategy refers to the added step of prehydrolysis by the addition of enzyme at 50 °C for 18 h before yeast cell inoculation.