Fig. 3.

Rad52 is necessary to maintain SAC activity. (A) Degradation kinetics of Cdc20 under nocodazole treatment. Samples were taken at the indicated time intervals. Hexokinase was used as a loading control. (B) Degradation kinetics of Pds1 under nocodazole treatment. An immunoblot assay was performed using an anti-myc antibody (Left). The highest value in each trial was used as the 100% standard to normalize the relative amounts of Pds1 (Right). All data in this figure represent the mean ± SD of triplicate experiments.