Fig. 2.

A representative Ca²⁺ trace for a single osteocyte before (dashed line) and after the start of cyclical loading (solid line) at 1 Hz to 3,000 $\mu ϵ$. Before mechanical loading, all osteocytes exhibited low-level Ca²⁺ fluctuations. Immediately following the start of mechanical loading, responsive cells showed cyclic increases in Ca²⁺ fluorescence intensity coinciding with the applied loading frequency.