Fig. S9.
SCG10 interacts with WDR47. (A) Colocalization images of WDR47 with SCG10 and SCG10 with JNK1. (B) Relative expression of SCG10 transcripts in n = 3 Wdr47tm1a/tm1a mice using qRT-PCR compared with WT across four tissues (cortex, spinal cord, thalamus, and liver) plotted as mean + SEM. (C) Colocalization of SCG10 and WDR47 in GT1-7 hypothalamic neuronal cells. Upper Left shows expression in GT1-7 cells transfected with pEYFP-WDR47 (green). Upper Right shows expression of SCG10 labeled with Texas red anti-SCG10 antibody (red). Lower Left is the overlay of Upper, and Lower Right is the 3D colocalization of WDR47 and SCG10. (D) Western blot analysis of SCG10 relative protein levels in response to WDR47 siRNA treatment. GAPDH is used as a loading control. Statistical analysis was done using Student’s t test (two-tailed). *P < 0.05. (Magnification: A, Top and Bottom, 20×/one dry objective; A, Middle, 65×/one oil objective.)