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. 2017 Oct 12;114(44):E9308–E9317. doi: 10.1073/pnas.1713625114

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Fig. S11. — WDR47 and LIS1 localization in yeast. (A) Living WT yeast cells (BY4742) expressing or overexpressing human LIS1-GFP or WDR47-GFP observed by fluorescence microscopy after staining of the vacuoles with the FM4-64 lipid dye. Merge shows the merge between the GFP and DsRED images, and Merge + DIC shows the merge between the GFP, DsRED, and DIC images. (B) Living WT yeast cells (BY4742) expressing human LIS1-GFP or WDR47-GFP observed by fluorescence microscopy to study localization in endosomal compartment positive for FYVE-DsRED (a probe for PtdIns3P that is localized on endosomes in yeast) and the Golgi complex (labeled with Sec7). (Magnification: 100×/1.45 oil objective.)