Fig. 4.
Site-directed RNA editing increases MeCP2 protein levels. (Top) Representative Western blot of whole-cell lysates from Mecp2R106Q/y or wild-type (WT, Mecp2+/y) sibling hippocampal neurons (DIV14) transduced 7 d earlier with AAV1/2 expressing either Editase alone or Editase and guide. The guide contains a C mismatch at the R106Q site and a G mismatch at the off-target A, T105T. (Bottom) Quantification of Western blots (mean ± SD, n = 3), each condition normalized to β-actin. Light-gray bar, cells transduced with Editase alone; dark-gray bar, cells transduced with Editase and guide. ***P < 0.001 by unpaired two-tailed t test.