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Genotyping of Mecp2R106Q mice. Representative agarose gel showing PCR amplicons from Mecp2+/y (lane 1), Mecp2R106Q/+ (lane 2), and Mecp2R106Q/y (lane 3) mice. Genomic DNA was extracted from ear tissue and PCR was performed on a region in Mecp2 intron 3 (primer sequences in SI Materials and Methods and Table S1).
